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1.
Genet Mol Res ; 14(1): 134-44, 2015 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-25729944

RESUMO

The aim of this study was to determine the Al concentration and the period of exposure of the roots of maize hybrids in minimal solution for efficient selection of genotypes that are Al-tolerant. Two experiments were performed (48 and 96 h of exposure) with increasing doses of Al in minimal solution; the block design was completely randomized in a split-plot design with 3 replications. By assessing differences in root growth (cm) and the percentage of inhibition of the growth of the main root (%), a marked decrease was observed in maize root growth with increasing Al concentration in the solution. Exposure of the roots to 2 mg/L Al for 48 h in minimal solution was the most efficient for selecting sources of tolerance, particularly for the hybrids H 44 and H 38.


Assuntos
Adaptação Fisiológica/efeitos dos fármacos , Alumínio/toxicidade , Hibridização Genética/efeitos dos fármacos , Seleção Genética/efeitos dos fármacos , Zea mays/genética , Zea mays/fisiologia , Análise de Variância , Raízes de Plantas/efeitos dos fármacos , Raízes de Plantas/crescimento & desenvolvimento , Análise de Regressão , Soluções , Zea mays/efeitos dos fármacos
2.
Genet Mol Res ; 12(1): 99-114, 2013 Jan 22.
Artigo em Inglês | MEDLINE | ID: mdl-23359029

RESUMO

Maize landraces derived from tropical germplasm represent an important source of genetic variability, which is currently poorly understood and under-exploited by Brazilian crop breeding programs. The aims of our study were to a) estimate the genetic diversity across 48 varieties of maize landraces cultivated at different locations in the States of Rio Grande do Sul (RS) and Paraná (PR) by means of random amplified polymorphic DNA (RAPD), simple sequence repeat (SSR), and amplified fragment length polymorphism (AFLP) markers; b) cluster these varieties based on their genetic similarity estimates, and c) establish possible correlations between genetic similarity and germplasm collection sites. Maize landrace accessions were genotyped through the 30 RAPD, 47 SSR, and 25 combinations of AFLP primers. The results revealed high levels of variability across landraces within and between collection sites. AFLP analysis resulted in amplification of 762 polymorphic fragments and a polymorphic index of 40.3%, followed by RAPD with 335 fragments (81.9%) and SSR with 105 fragments (78.3%). The genetic similarity estimates of the investigated landraces ranged from 41 (SSR) to 74% (AFLP), and the amplitudes of these indices were notably similar between RAPD and SSR, as well as between AFLP and joint analysis. Regarding the RAPD and AFLP dendrograms, groups comprising accessions from RS prevailed, whereas SSR comprised varieties from both collection sites. Groups exclusive to RS or PR support the hypothesis that divergence between groups is possible owing to the fixation of regional adaptation alleles and to spatial barriers hindering genetic flow between locations.


Assuntos
Genes de Plantas , Repetições de Microssatélites , Zea mays/genética , Análise do Polimorfismo de Comprimento de Fragmentos Amplificados/métodos , Biomarcadores , Brasil , Cruzamento/métodos , Variação Genética , Genótipo , Polimorfismo Genético , Técnica de Amplificação ao Acaso de DNA Polimórfico/métodos
3.
Neuroscience ; 228: 349-60, 2013 Jan 03.
Artigo em Inglês | MEDLINE | ID: mdl-23111126

RESUMO

Handedness is most often measured by questionnaires that assess an individual's preference for using a particular hand to perform a variety of tasks. While such assessments have proved reliable, they do not address the underlying neurobehavioral processes that give rise to the choice of which hand to use. Recent research has indicated that handedness is associated with hemispheric specializations for different aspects of sensorimotor performance. We now hypothesize that an individual's choice of which hand to use for a given task should result from an interaction between these underlying neurobehavioral asymmetries with task conditions. We test this hypothesis by manipulating two factors in targeted reaching movements: (1) region of workspace and (2) visual feedback conditions. The first manipulation modified the geometric and dynamic requirements of the task for each arm, whereas the second modified the sensorimotor performance asymmetries, an effect predicted by previous literature. We expected that arm choice would be reflected by an interaction between these factors. Our results indicated that removing visual feedback both improved the relative performance of the non-dominant arm and increased the choice to use this arm for targets near midline, an effect that was enhanced for targets requiring larger movement amplitudes. We explain these findings in the context of the dynamic dominance hypothesis of handedness and discuss their implications for the link between hemispheric asymmetries in neural control and hand preference.


Assuntos
Comportamento de Escolha/fisiologia , Lateralidade Funcional/fisiologia , Desempenho Psicomotor/fisiologia , Adolescente , Adulto , Feminino , Previsões , Humanos , Masculino , Estimulação Luminosa/métodos , Adulto Jovem
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